improvement of leishmania major amastigote elisa test using various antigen antibody dissociating agents and pepsin digestion.

Improvement of Leishmania major Amastigote ELISA Test Using Various Antigen Antibody Dissociating Agents and Pepsin Digestion.

Improvement of Leishmania major Amastig~te ELISA Test Using Various Antigen-Antibody Dissociating Agents and Pepsin Digestion

Amastigotes arc main pathogenic stage of I.eishmania parasite in mammalian host. During the course of leishmania infection the amastigotc surface would be covered by host proteins like immunoglobulins and complement components. These proteins produce false positive reaction and low signal/noise ratio in evaluation of antibody raising against lesion-dcrived amastigotc by indirect ELISA. 1'0 over...

Detection of Leishmania major using PCR-ELISA

Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorben...

The structure of Leishmania major amastigote lipophosphoglycan.

Intracellular amastigotes of Leishmania major produce 6 x 10(4) copies/cell of a lipophosphoglycan (LPG) that is structurally distinct from the LPG produced by the extracellular promastigote form of L. major, Leishmania donovani, and Leishmania mexicana (reviewed by McConville, M. J. (1991) Cell Biol. Int. Rep. 15, 779-798). L. major amastigote LPG is composed of a lysoalkyl phosphatidylinosito...

Development of an ELISA Test for Detection of Anti FMD Virus Type A87/IRN Antibody Using Irradiated Antigen

Background and Aims: The aim of this study was application of gamma irradiated FMDV antigen for liquid phase blocking sandwich ELISA (B-ELISA) to quantify guinea pig anti FMDV type A87/IRN antibody. Methods: FMDV type A87/IRN was propagated to a titer of 107.5/ml TCID50 and then irradiated by gamma irradiator. The irradiated 146 S antigen was purified by sucrose gradient centrifugation after in...

Partial Purification of a Potent Immunosuppressive Factor Excreted from Leishmania major Promastigote and Amastigote

Recent scientific evidence indicates that distinct patterns of susceptibility in BALB/c mice to Leishmania major infection are attributable to the differential expansion of distinct CD4+ T-cell subsets and their cytokines production. Production of the Th1 cytokine IFN-g is associated with resistance, whereas production of the Th2 cytokines IL-4 and IL-10 are associated with extreme susceptibili...